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InhaltsangabeI Considerations for Routine Imaging.- 1 Entering the Portal: Understanding the Digital Image Recorded Through a Microscope.- 2 Quantitative Biological Image Analysis.- 3 The Open Microscopy Environment: a Collaborative Data Modeling and Software Development Project for Biological Image Informatics.- 4 Design and Function of a Light-Microscopy Facility.- II Advanced Methods and Concepts.- 5 Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls.- 6 Quantitative FRET Microscopy of Live Cells.- 7 Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells.- 8 Single Fluorescent Molecule Tracking in Live Cells.- 9 From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins. III CuttingEdge Applications and Utilities. 10 Towards Imaging the Dynamics of Protein Signalling. 11 New Technologies for Imaging and Analysis of Individual Microbial Cells. 12 Imaging Parasites in Vivo. 13 ComputerAssisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells. 14 HighThroughput/HighContent Automated Image Acquisition and Analysis. 15 Cognition Network Technology a Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents. 16 HighContent Phenotypic CellBased Assays.

'Imaging cellular and molecular biological function' provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.