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InhaltsangabePrefaceB. Shapiro* and M. Hofreiter Chapter 1:  Setting Up An Ancient DNA LaboratoryT. L. Fulton* Chapter 2: A Phenol-Chloroform Protocol for Extracting DNA from Ancient SamplesR. Barnett* and G. Larson Chapter 3: DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica ParticlesN. Rohland* Chapter 4: Case Study: Recovery of ancient nuclear DNA from Toe Pads of the Extinct Passenger Pigeon  T. L. Fulton*, S. M. Wagner and B. Shapiro Chapter 5: Extraction of DNA from PaleofecesM. Kuch and H. Poinar* Chapter 6: DNA Extraction from Keratin and ChitinP. F. Campos, M. T. P. Gilbert* Chapter 7: Case Study:  Ancient sloth DNA Recovered from Hairs Preserved in Paleofeces A.A. Clack*, R.D.E. MacPhee, H.N. Poinar Chapter 8: Ancient DNA Extraction from Soils and SedimentsJ. Haile* Chapter 9: DNA Extraction from Fossil EggshellC. L. Oskam and M. Bunce* Chapter 10: Ancient DNA Extraction from PlantsL. Kistler* Chapter 11: DNA Extraction from Formalin-fixed MaterialP. F. Campos, M. T. P. Gilbert* Chapter 12: Case Study: Ancient DNA recovered from Pleistocene-age Remains of a Florida Armadillo B. Letts and B. Shapiro* Chapter 13: Non-destructive DNA Extraction from Museum SpecimensM. Hofreiter* Chapter 14: Case Study: Using a Non-destructive DNA Extraction Method to Generate mtDNA Sequences from Historical Chimpanzee Specimens E. Mohandesan, S. Prost, M. Hofreiter* Chapter 15: PCR Amplification, Cloning and Sequencing of Ancient DNAT. L. Fulton* and M. Stiller Chapter 16: Quantitative Real-time PCR in aDNA Research M. Bunce*, C. L. Oskam, M. E. Allentoft Chapter 17: Multiplex PCR Amplification of Ancient DNAM. Stiller*, T. L. Fulton Chapter 18: Preparation of Next-generation Sequencing Libraries from Damaged DNAA. W. Briggs*, P. Heyn Chapter 19: Generating Barcoded Libraries for Multiplex High-throughput Sequencing  M. Knapp*, M. Stiller, M. Meyer Chapter 20: Case Study: Targeted high-throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS)M. Stiller* Chapter 21: Target Enrichment via DNA Hybridization CaptureS. Horn* Chapter 22: Case Study: Enrichment of Ancient Mitochondrial DNA by Hybridization Capture  S. Horn* Chapter 23: Analysis of High-throughput Ancient DNA Sequencing DataM. Kircher* Chapter 24: Phylogenetic Analysis of Ancient DNA using BEASTS. Y. W. Ho* E. Mohandesan, S. Prost, M. Hofreiter* Chapter 15: PCR Amplification, Cloning and Sequencing of Ancient DNAT. L. Fulton* and M. Stiller Chapter 16: Quantitative Real-time PCR in aDNA Research M. Bunce*, C. L. Oskam, M. E. Allentoft Chapter 17: Multiplex PCR Amplification of Ancient DNAM. Stiller*, T. L. Fulton Chapter 18: Preparation of Next-generation Sequencing Libraries from Damaged DNAA. W. Briggs*, P. Heyn Chapter 19: Generating Barcoded Libraries for Multiplex High-throughput Sequencing  M. Knapp*, M. Stiller, M. Meyer Chapter 20: Case Study: Targeted high-throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS)M. Stiller* Chapter 21: Target Enrichment via DNA Hybridization CaptureS. Horn* Chapter 22: Case Study: Enrichment of Ancient Mitochondrial DNA by Hybridization Capture  S. Horn* Chapte

Closes the gap between appropriate methodologicol approaches and retrieval and anysis of ancient DNAProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts