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Biofilm biology has become an expanding field of research in human, industrial and environmental ecosystems.On surveying the endodontic literature it is obvious that this realization and the fact that biofilms afford the resident microorganisms protection/resistance against harmful exogeneous influences including antimicrobial agents, is rather new to endodontology. Hence, the conditions under which biofilms occur in endodontic infections, and the measures that ought to be taken for their eradication, are not well understood. In this book the biofilm concept is presented and how it may apply to endodontic infections is discussed.